StemBeads FGF2

$125.00$289.00

A media additive designed to reduce feeding times and improve the quality of FGF2 dependent cell cultures.

$125.00
$289.00

Description

StemBeads® FGF2 is a patented growth factor supplement that offers a novel way to culture cells with native Fibroblast Growth Factor 2 more efficiently. FGF2 is key for growth of human induced pluripotent stem cells (iPSCs), fibroblast cells, wound healing cells such as macrophages, cancer cells, and neural progenitor cells (NPCs). StemBeads® FGF2 are microbeads composed of an FDA approved, biodegradable PLGA polymer that is loaded with native recombinant human FGF2. Controlled delivery and stable levels overcome the 4.5 hour half-life (Figure above) of FGF2 and improve cell cultures while saving researchers valuable time and resources.

StemBeads® FGF2 have been tested in media such as mTeSR, mTeSR plus, Stemflex, Flex 8, Nutristem, 10% FBS, and neuronal medium including neural progenitor expansion medium (NPEM) with enhanced cellular profiles. StemBeads® FGF2 can be combined with other StemBeads® varieties.

StemBeads FGF2 Product Information Sheet
Application Note: Using StemBeads® FGF2 in Defined Media
Frequently Asked Questions
SDS – StemBeads® FGF2

Sustained Release Protein Cell Culture

StemBeads are a revolutionary controlled-release growth factor technology. The StemBeads product is currently used for improved cell culture in more than 100 scientific laboratories globally. StemBeads technology encapsulates a growth factor in a PLGA hydrogel that stabilizes the protein and releases it steadily over several days. With growth factor levels stabilized, spontaneous differentiation is reduced, pluripotency is increased, and feeding schedules are reduced to only 2-3 times per week.

StemBeads are added to your medium to achieve a desired growth factor release, sustained over several days. Following, the beads are washed from the culture, the medium is replaced, and the process repeated as required.

4 reviews for StemBeads FGF2

  1. David

    We have been using StemBeads FGF2 for ~2 years on our iPSCs. Our cultures have very little spontaneous differentiation with a significant reduction of media usage. I could not imagine going back to daily feeding!

  2. Emily

    I use StemBeads FGF2 on both ESCs and iPSCs and the cultures look great with little differentiation. I have been using the beads for a little under a year and I have found them very consistent and reliable. Incorporating the StemBeads FGF2 into the media is such a quick and simple procedure and produces high quality cultures while saving both time and money. Not having to feed every day and having great quality cultures is the best of both!

  3. Evan

    I help maintain several different iPSC lines, which can be quite time consuming. Working with FGF2 beads from StemCultures significantly cuts down on my feeding time, and allows me to focus on other tasks! Moreover, when an experiment calls for a high cell confluency, I can rely on this product to hold back the spontaneous differentiation.

  4. Charles

    Our lab has been exclusively using StemBeads as our standard protocol for maintaining, expanding, and differentiating hiPSCs and hESCs for over 4 years. StemBeads provide a much higher level of control of our cell cultures while maximizing efficiency within the lab e.g. decreased labor via eliminating daily media changes/feeds. Highly recommend StemBeads to anyone who values their time and the quality of their cultures!

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