Description
For an instructional video on how to use DISCs, click here.
*NOTE: For 2D cultures, place DISC in a transwell to avoid cell lifting. For 3D cultures (i.e. organoids) place DISC directly in the culture well.
BDNF and GDNF DISCs are patented inert, non-degradable, biocompatible hydrogels that release defined levels of native BDNF or GDNF stably into culture medium over two weeks. DISCs are loaded with StemBeads®, PLGA microbeads that encapsulate the protein. BDNF and GDNF are key for supporting neuronal differentiation. DISCs are easy to add and remove, giving scientists enhanced control of growth factor levels in their cultures. Controlled delivery and stable levels overcome the 45-minute or 2 hour half-life of these factors while saving researchers valuable time and resources, allowing the following:
- Stable growth factor levels.
- Known growth factor levels.
- Less media and labor cost.
- Increase MAP2A-positive axonal projections.
- Enhanced dendritic arborization.
- Increased Synapsin1 co-localization with MAP2A positive cortical neurons.
BDNF and GDNF DISCs have been tested in multiple neuronal base media, including Brain Phys, with enhanced cellular profiles. In these cases, BDNF DISCs were used with and without GDNF DISCs.
We recommend a release of 10 ng/mL when culturing neuronal cells. However, to fit other needs, the release can be adjusted slightly based on the amount of medium and the number of DISCs that are added. See the chart below for reference.
| Number of DISCs Per Well | Volume of Medium Added Per Well | Sustained BDNF or GDNF Release |
| 1 | 2 mL | 5 ng/mL |
| 1 | 1 mL | 10 ng/mL |
| 1 | 0.5 mL | 20 ng/mL |
| 2 | 2 mL | 10 ng/mL |
| 3 | 2 mL | 15 ng/mL |
| 4 | 2 mL | 20 ng/mL |
DISC device technology allows you to use your favorite media and feed less often. One DISC can remain in the culture well/vessel for several media feeds (i.e., a pack of 12 DISCs will last 4 weeks when culturing in a 6 well plate). When added into media, DISCs are designed to release at a constant 5 ng/mL. As DISCs™ rehydrate, they will swell and become transparent. Embedded StemBeads® will be visible under a microscope. This technology gives scientists enhanced control over growth factor levels in their cultures to provide robust, high-quality cells.
Product Information Sheet BDNF DISCs
Frequently Asked Questions BDNF DISCs
SDS – BDNF DISCs
Product Information Sheet GDNF DISCs
Frequently Asked Questions GDNF DISCs
SDS – GDNF DISCs
