Description
For an instructional video on how to use DISCs, click here.
*NOTE: For 2D cultures, place DISC in a transwell to avoid cell lifting. For 3D cultures (i.e. organoids) place DISC directly in the culture well.
GDNF DISCs are patented inert, non-degradable, biocompatible hydrogels that release defined levels of native GDNF stably into culture medium over the course of two weeks. GDNF DISCs are loaded with StemBeads® GDNF, PLGA microbeads that encapsulate the protein. GDNF is key for supporting neuronal differentiation. GDNF DISCs are easy to add and remove, giving scientists enhanced control of growth factor levels in their cultures. Controlled delivery and stable levels overcome the 45-minute half-life of GDNF and improve cell cultures while saving researchers valuable time and resources, allowing the following:
- Stable growth factor levels.
- Known growth factor levels.
- Less media and labor costs.
- Increase MAP2A-positive axonal projections.
- Enhanced dendritic arborization.
- Increased Synapsin1 co-localization with MAP2A positive cortical neurons.
GDNF DISCs have been tested in multiple neuronal base media, including Brain Phys, with enhanced cellular profiles. In these cases, GDNF DISCs were used with and without BDNF DISCs.
We recommend a release of 5 ng/mL when culturing neuronal cells. However, to fit other needs, the release can be adjusted slightly based on the amount of medium and the number of DISCs that are added. See the chart below for reference.
| Number of DISCs Per Well | Volume of Medium Added Per Well |
Sustained GDNF Release |
| 1 | 2 mL | 5 ng/mL |
| 1 | 1 mL | 10 ng/mL |
| 1 | 0.5 mL | 20 ng/mL |
| 2 | 2 mL | 10 ng/mL |
| 3 | 2 mL | 15 ng/mL |
| 4 | 2 mL | 20 ng/mL |
DISC device technology allows you to use your favorite media and feed less often. One DISC can remain in the culture well/vessel for several media feeds (i.e., a pack of 12 DISCs will last 4 weeks when culturing in a 6 well plate). When added into media, DISCs are designed to release at a constant 5 ng/mL. As DISCs™ rehydrate, they will swell and become transparent. Embedded StemBeads® will be visible under a microscope. This technology gives scientists enhanced control over growth factor levels in their cultures to provide robust, high-quality cells.
Product Information Sheet GDNF DISCs
Frequently Asked Questions GDNF DISCs
SDS – GDNF DISCs
