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stem cell growth factors

Qkine BDNF DISCs

$90.00

USE: Add one DISC into one well of a 6-well plate, change DISC once every two weeks and feed with your regular medium 2-3 times a week.*

 

$90.00

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Forceps
19 in stock
Forceps
We recommend using a pair of sterile, curved forceps, similar to the ones shown, to add DISCs to your culture wells. These forceps are not required to purchase DISCs, but can be added to your order for your convenience.
Price $20.00

Description

For an instructional video on how to use DISCs, click here.

*NOTE: For 2D cultures, place DISC in a transwell to avoid cell lifting. For 3D cultures (i.e. organoids) place DISC directly in the culture well.

Qkine BDNF DISCs are patented inert, non-degradable, biocompatible hydrogels that release defined levels of Qkine recombinant human BDNF (Qk050) stably into culture medium over two weeks. Qkine BDNF DISCs are loaded with StemBeads® Qkine BDNF (Qk-SBBD1), PLGA microbeads that encapsulate the protein. BDNF is key for supporting neuronal differentiation. BDNF DISCs are easy to add and remove, giving scientists enhanced control of growth factor levels in their cultures. Controlled delivery and stable levels overcome the 2 hour half-life of BDNF and improve cell cultures while saving researchers valuable time and resources, allowing the following:

  • Stable growth factor levels.
  • Known growth factor levels.
  • Less media and labor cost.
  • Increase MAP2A-positive axonal projections.
  • Enhanced dendritic arborization.
  • Increased Synapsin1 co-localization with MAP2A positive cortical neurons.

Qkine BDNF DISCs have been tested in multiple neuronal base media, including Brain Phys, with enhanced cellular profiles. In these cases, Qkine BDNF DISCs were used with and without Qkine GDNF DISCs.

We recommend a release of 5 ng/mL when culturing neuronal cells. However, to fit other needs, the release can be adjusted slightly based on the amount of medium and the number of DISCs that are added. See the chart below for reference.

Number of DISCs Per Well Volume of Medium Added Per Well

Sustained BDNF Release
1 2 mL 5 ng/mL
1 1 mL 10 ng/mL
1 0.5 mL 20 ng/mL
2 2 mL 10 ng/mL
3 2 mL 15 ng/mL
4 2 mL 20 ng/mL

DISC device technology allows you to use your favorite media and feed less often. One DISC can remain in the culture well/vessel for several media feeds (i.e., a pack of 12 DISCs will last 4 weeks when culturing in a 6 well plate). When added into media, DISCs are designed to release at a constant 5 ng/mL. As DISCs™ rehydrate, they will swell and become transparent. Embedded StemBeads® will be visible under a microscope. This technology gives scientists enhanced control over growth factor levels in their cultures to provide robust, high-quality cells.

Product Information Sheet Qkine BDNF DISCs
Frequently Asked Questions BDNF DISCs
SDS – Qkine BDNF DISCs

Additional information

Forceps

No, Yes

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