FGF2 DISCs

$78.00$288.00

USE: Add one DISC into one well of a 6-well plate, change DISC once a week and feed with your regular medium 2-3 times a week. 

Note: Standard size DISCs release at 10 ng/mL in 2 mL of medium; Mini size DISCs release at 10 ng/mL in 1 mL of medium. See table below to determine which DISC size suits your needs.

$78.00
$288.00
$216.00

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Forceps
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Forceps
We recommend using a pair of sterile, curved forceps, similar to the ones shown, to add DISCs to your culture wells. These forceps are not required to purchase DISCs, but can be added to your order for your convenience.
Price $20.00

Description

USE: Add one DISC into one well of a 6-well plate, change DISC once a week and feed with your regular medium 2-3 times a week.

For an instructional video on how to use FGF2 DISCs, click here.

FGF2 DISCs are biocompatible hydrogels that release defined levels of native FGF2 stably into culture medium over the course of one week. Native FGF2 has a short half life (4 hours) and rapidly decays in cell culture media. Adding soluble FGF2 through daily, conventional commercial media feeds creates fluctuations in growth factor levels that affect signaling pathways and creates a stressful cellular environment. With FGF2 DISCs, FGF2 levels are stable over one week, allowing the following:

  • Reduced feeding schedule to 2-3 times a week and improved pluripotency
  • Dampened heterogeneity between cell lines
  • Improved directed differentiation efficiency into endoderm, mesoderm, and ectoderm lineages
  • Compatibility with different media

DISC device technology allows you to use your favorite media and feed less often. One DISC can be added per passage and can remain in the culture well/vessel for several media feeds (i.e., a pack of 48 DISCs will last 2 months when culturing in a 6 well plate). When added into media, DISCs are designed to release at a constant 10 ng/mL. As DISCs™ rehydrate, they will swell and become transparent. Embedded StemBeads® will be visible under a microscope. An image of a DISC™ under a microscope is included above for reference. This technology gives scientists enhanced control over growth factor levels in their cultures to provide robust, high-quality cells.

StemCultures offers two product sizes to accommodate standard culture plate sizes. Standard size DISCs (DSC500-48, DSC500S) are approximately 2-3 mm in diameter when dry, 5-6 mm diameter when rehydrated. Mini DISCs (DSC505-48) are approximately 1-2 mm in diameter when dry, 2-3 mm in diameter when rehydrated. To find which DISC is ideal for your purpose, see the table below.

Plate Size

Volume of Medium Added Recommended DISC Release in Volume of Medium Added*

6 well

2 mL Standard (DSC500) 10 ng/mL
12 well 1 mL Mini (DSC505)

10 ng/mL

12 well 2 mL Standard (DSC500)

10 ng/mL

24 well 1 mL Mini (DSC505)

10 ng/mL

24 well 2 mL Standard (DSC500)

10 ng/mL

48 well 1 mL Mini (DSC505)

10 ng/mL

*we recommend a release of 10 ng/mL when culturing iPSCs. However, to fit other needs, the release can be adjusted slightly based on the amount of media that is added. See the chart below for reference.

Number of Standard Size DISCs (DSC500) Per Well

Volume of Medium Added Per Well Sustained FGF2 Release

1 DISC

250 uL

80 ng/mL

1 DISC 500 uL

40 ng/mL

1 DISC

1 mL

20 ng/mL

1 DISC

2 mL

10 ng/mL

1 DISC 4 mL

5 ng/mL

Product Information Sheet FGF2 DISCs Standard
Product Information Sheet FGF2 DISCs Mini
Frequently Asked Questions FGF2 DISCs

Sustained Release Protein Cell Culture

StemBeads are a revolutionary controlled-release growth factor technology. The StemBeads product is currently used for improved cell culture in more than 100 scientific laboratories globally. StemBeads technology encapsulates a growth factor in a PLGA hydrogel that stabilizes the protein and releases it steadily over several days. With growth factor levels stabilized, spontaneous differentiation is reduced, pluripotency is increased, and feeding schedules are reduced to only 2-3 times per week.

StemBeads are added to your medium to achieve a desired growth factor release, sustained over several days. Following, the beads are washed from the culture, the medium is replaced, and the process repeated as required.

Additional information

Forceps

No, Yes

6 reviews for FGF2 DISCs

  1. Steve Lotz – Director of NeuraCell

    I am currently using the FGF2 DISCs on all my iPSC cultures. I have about 33 different lines going for multiple things like expanding for making new banks, organoid production, NPC and RPE differentiations. The DISCs really make a huge difference in the quality of my cells and make the workload much easier to handle.

  2. Susan Goderie – Laboratory Manager at the Neural Stem Cell Institute

    Discs save time and money both of which are needed when doing research. The quality of our iPSCs, especially the “tricky” lines, has improved significantly. One big advantage using the discs, is being able to remove all growth factors by simply pipetting the disc off the cultures! We have even been able to timelapse image live cells with discs. The discs are worth trying!

  3. Jade Bola – Lab Technician at the Neural Stem Cell Institute

    The FGF2 discs are a really great addition to my cell culture work with iPSCs. Previously, our iPSCs would have to be fed every single day. With the discs however, the discs last a whole week and media only has to be changed a couple times a week. This saves us from using up so much media, which can get expensive, and saves us a lot of time! I’ve also noticed that with the discs, our iPSC cultures land better, grow faster, and spontaneously differentiate much less. I highly recommend them to anyone!

  4. Dong Eun Seo – Graduate Student at University of Georgia

    With FGF2 DISCs, almost 100% of cells highly express pluripotency markers. Also, I could save a lot of media since I have to change media every 2 to 3 days. I’m planning to keep using these FGF2 DISCs for differentiation assays.

  5. Christopher Taitano-Johnson – Laboratory Technician at UCLA

    Great product, great service/communication with fast delivery.

  6. Kristen Whitney – Icahn School of Medicine at Mount Sinai

    The FGF2 discs are a huge improvement to our stem cell pipeline. With the discs spontaneous differentiation is almost completely eliminated, and pluripotency markers expression has increased to nearly 100% which is critical for our organoid experiments. Highly recommend!

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